Mass spectrometric characterization of proteins from the SARS virus: a preliminary report.
Identifieur interne : 005F37 ( Main/Exploration ); précédent : 005F36; suivant : 005F38Mass spectrometric characterization of proteins from the SARS virus: a preliminary report.
Auteurs : Oleg Krokhin [Canada] ; Yan Li ; Anton Andonov ; Heinz Feldmann ; Ramon Flick ; Steven Jones ; Ute Stroeher ; Nathalie Bastien ; Kumar V N. Dasuri ; Keding Cheng ; J Neil Simonsen ; Hélène Perreault ; John Wilkins ; Werner Ens ; Frank Plummer ; Kenneth G. StandingSource :
- Molecular & cellular proteomics : MCP [ 1535-9476 ] ; 2003.
Descripteurs français
- KwdFr :
- Alignement de séquences, Animaux, Données de séquences moléculaires, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (analyse), Glycoprotéines membranaires (immunologie), Humains, Masse moléculaire, Protéines de l'enveloppe virale (analyse), Protéines de l'enveloppe virale (immunologie), Protéines nucléocapside (analyse), Protéines nucléocapside (immunologie), Protéines virales (analyse), Protéines virales (immunologie), Spectrométrie de masse MALDI (), Syndrome respiratoire aigu sévère (diagnostic), Séquence d'acides aminés, Virus du SRAS ().
- MESH :
- analyse : Glycoprotéines membranaires, Protéines de l'enveloppe virale, Protéines nucléocapside, Protéines virales.
- diagnostic : Syndrome respiratoire aigu sévère.
- immunologie : Glycoprotéines membranaires, Protéines de l'enveloppe virale, Protéines nucléocapside, Protéines virales.
- Alignement de séquences, Animaux, Données de séquences moléculaires, Glycoprotéine de spicule des coronavirus, Humains, Masse moléculaire, Spectrométrie de masse MALDI, Séquence d'acides aminés, Virus du SRAS.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Humans, Membrane Glycoproteins (analysis), Membrane Glycoproteins (immunology), Molecular Sequence Data, Molecular Weight, Nucleocapsid Proteins (analysis), Nucleocapsid Proteins (immunology), SARS Virus (chemistry), Sequence Alignment, Severe Acute Respiratory Syndrome (diagnosis), Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization (methods), Spike Glycoprotein, Coronavirus, Viral Envelope Proteins (analysis), Viral Envelope Proteins (immunology), Viral Proteins (analysis), Viral Proteins (immunology).
- MESH :
- chemical , analysis : Membrane Glycoproteins, Nucleocapsid Proteins, Viral Envelope Proteins, Viral Proteins.
- chemical , immunology : Membrane Glycoproteins, Nucleocapsid Proteins, Viral Envelope Proteins, Viral Proteins.
- chemistry : SARS Virus.
- diagnosis : Severe Acute Respiratory Syndrome.
- methods : Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization.
- Amino Acid Sequence, Animals, Humans, Molecular Sequence Data, Molecular Weight, Sequence Alignment, Spike Glycoprotein, Coronavirus.
Abstract
A new coronavirus has been implicated as the causative agent of severe acute respiratory syndrome (SARS). We have used convalescent sera from several SARS patients to detect proteins in the culture supernatants from cells exposed to lavage another SARS patient. The most prominent protein in the supernatant was identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) as a approximately 46-kDa species. This was found to be a novel nucleocapsid protein that matched almost exactly one predicted by an open reading frame in the recently published nucleotide sequence of the same virus isolate (>96% coverage). A second viral protein corresponding to the predicted approximately 139-kDa spike glycoprotein has also been examined by MALDI-TOF MS (42% coverage). After peptide N-glycosidase F digestion, 12 glycosylation sites in this protein were confirmed. The sugars attached to four of the sites were also identified. These results suggest that the nucleocapsid protein is a major immunogen that may be useful for early diagnostics, and that the spike glycoprotein may present a particularly attractive target for prophylactic intervention in combating SARS.
DOI: 10.1074/mcp.M300048-MCP200
PubMed: 12775768
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 003371
- to stream PubMed, to step Curation: 003371
- to stream PubMed, to step Checkpoint: 003185
- to stream Ncbi, to step Merge: 000122
- to stream Ncbi, to step Curation: 000122
- to stream Ncbi, to step Checkpoint: 000122
- to stream Main, to step Merge: 006424
- to stream Main, to step Curation: 005F37
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Mass spectrometric characterization of proteins from the SARS virus: a preliminary report.</title><author><name sortKey="Krokhin, Oleg" sort="Krokhin, Oleg" uniqKey="Krokhin O" first="Oleg" last="Krokhin">Oleg Krokhin</name><affiliation wicri:level="4"><nlm:affiliation>Department of Physics, University of Manitoba, Winnipeg MB R3T 2N2, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Physics, University of Manitoba, Winnipeg MB R3T 2N2</wicri:regionArea><orgName type="university">Université du Manitoba</orgName><placeName><settlement type="city">Winnipeg</settlement><region type="state">Manitoba</region></placeName></affiliation></author><author><name sortKey="Li, Yan" sort="Li, Yan" uniqKey="Li Y" first="Yan" last="Li">Yan Li</name></author><author><name sortKey="Andonov, Anton" sort="Andonov, Anton" uniqKey="Andonov A" first="Anton" last="Andonov">Anton Andonov</name></author><author><name sortKey="Feldmann, Heinz" sort="Feldmann, Heinz" uniqKey="Feldmann H" first="Heinz" last="Feldmann">Heinz Feldmann</name></author><author><name sortKey="Flick, Ramon" sort="Flick, Ramon" uniqKey="Flick R" first="Ramon" last="Flick">Ramon Flick</name></author><author><name sortKey="Jones, Steven" sort="Jones, Steven" uniqKey="Jones S" first="Steven" last="Jones">Steven Jones</name></author><author><name sortKey="Stroeher, Ute" sort="Stroeher, Ute" uniqKey="Stroeher U" first="Ute" last="Stroeher">Ute Stroeher</name></author><author><name sortKey="Bastien, Nathalie" sort="Bastien, Nathalie" uniqKey="Bastien N" first="Nathalie" last="Bastien">Nathalie Bastien</name></author><author><name sortKey="Dasuri, Kumar V N" sort="Dasuri, Kumar V N" uniqKey="Dasuri K" first="Kumar V N" last="Dasuri">Kumar V N. Dasuri</name></author><author><name sortKey="Cheng, Keding" sort="Cheng, Keding" uniqKey="Cheng K" first="Keding" last="Cheng">Keding Cheng</name></author><author><name sortKey="Simonsen, J Neil" sort="Simonsen, J Neil" uniqKey="Simonsen J" first="J Neil" last="Simonsen">J Neil Simonsen</name></author><author><name sortKey="Perreault, Helene" sort="Perreault, Helene" uniqKey="Perreault H" first="Hélène" last="Perreault">Hélène Perreault</name></author><author><name sortKey="Wilkins, John" sort="Wilkins, John" uniqKey="Wilkins J" first="John" last="Wilkins">John Wilkins</name></author><author><name sortKey="Ens, Werner" sort="Ens, Werner" uniqKey="Ens W" first="Werner" last="Ens">Werner Ens</name></author><author><name sortKey="Plummer, Frank" sort="Plummer, Frank" uniqKey="Plummer F" first="Frank" last="Plummer">Frank Plummer</name></author><author><name sortKey="Standing, Kenneth G" sort="Standing, Kenneth G" uniqKey="Standing K" first="Kenneth G" last="Standing">Kenneth G. Standing</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2003">2003</date><idno type="RBID">pubmed:12775768</idno><idno type="pmid">12775768</idno><idno type="doi">10.1074/mcp.M300048-MCP200</idno><idno type="wicri:Area/PubMed/Corpus">003371</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">003371</idno><idno type="wicri:Area/PubMed/Curation">003371</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">003371</idno><idno type="wicri:Area/PubMed/Checkpoint">003185</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">003185</idno><idno type="wicri:Area/Ncbi/Merge">000122</idno><idno type="wicri:Area/Ncbi/Curation">000122</idno><idno type="wicri:Area/Ncbi/Checkpoint">000122</idno><idno type="wicri:doubleKey">1535-9476:2003:Krokhin O:mass:spectrometric:characterization</idno><idno type="wicri:Area/Main/Merge">006424</idno><idno type="wicri:Area/Main/Curation">005F37</idno><idno type="wicri:Area/Main/Exploration">005F37</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Mass spectrometric characterization of proteins from the SARS virus: a preliminary report.</title><author><name sortKey="Krokhin, Oleg" sort="Krokhin, Oleg" uniqKey="Krokhin O" first="Oleg" last="Krokhin">Oleg Krokhin</name><affiliation wicri:level="4"><nlm:affiliation>Department of Physics, University of Manitoba, Winnipeg MB R3T 2N2, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Physics, University of Manitoba, Winnipeg MB R3T 2N2</wicri:regionArea><orgName type="university">Université du Manitoba</orgName><placeName><settlement type="city">Winnipeg</settlement><region type="state">Manitoba</region></placeName></affiliation></author><author><name sortKey="Li, Yan" sort="Li, Yan" uniqKey="Li Y" first="Yan" last="Li">Yan Li</name></author><author><name sortKey="Andonov, Anton" sort="Andonov, Anton" uniqKey="Andonov A" first="Anton" last="Andonov">Anton Andonov</name></author><author><name sortKey="Feldmann, Heinz" sort="Feldmann, Heinz" uniqKey="Feldmann H" first="Heinz" last="Feldmann">Heinz Feldmann</name></author><author><name sortKey="Flick, Ramon" sort="Flick, Ramon" uniqKey="Flick R" first="Ramon" last="Flick">Ramon Flick</name></author><author><name sortKey="Jones, Steven" sort="Jones, Steven" uniqKey="Jones S" first="Steven" last="Jones">Steven Jones</name></author><author><name sortKey="Stroeher, Ute" sort="Stroeher, Ute" uniqKey="Stroeher U" first="Ute" last="Stroeher">Ute Stroeher</name></author><author><name sortKey="Bastien, Nathalie" sort="Bastien, Nathalie" uniqKey="Bastien N" first="Nathalie" last="Bastien">Nathalie Bastien</name></author><author><name sortKey="Dasuri, Kumar V N" sort="Dasuri, Kumar V N" uniqKey="Dasuri K" first="Kumar V N" last="Dasuri">Kumar V N. Dasuri</name></author><author><name sortKey="Cheng, Keding" sort="Cheng, Keding" uniqKey="Cheng K" first="Keding" last="Cheng">Keding Cheng</name></author><author><name sortKey="Simonsen, J Neil" sort="Simonsen, J Neil" uniqKey="Simonsen J" first="J Neil" last="Simonsen">J Neil Simonsen</name></author><author><name sortKey="Perreault, Helene" sort="Perreault, Helene" uniqKey="Perreault H" first="Hélène" last="Perreault">Hélène Perreault</name></author><author><name sortKey="Wilkins, John" sort="Wilkins, John" uniqKey="Wilkins J" first="John" last="Wilkins">John Wilkins</name></author><author><name sortKey="Ens, Werner" sort="Ens, Werner" uniqKey="Ens W" first="Werner" last="Ens">Werner Ens</name></author><author><name sortKey="Plummer, Frank" sort="Plummer, Frank" uniqKey="Plummer F" first="Frank" last="Plummer">Frank Plummer</name></author><author><name sortKey="Standing, Kenneth G" sort="Standing, Kenneth G" uniqKey="Standing K" first="Kenneth G" last="Standing">Kenneth G. Standing</name></author></analytic><series><title level="j">Molecular & cellular proteomics : MCP</title><idno type="ISSN">1535-9476</idno><imprint><date when="2003" type="published">2003</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Humans</term><term>Membrane Glycoproteins (analysis)</term><term>Membrane Glycoproteins (immunology)</term><term>Molecular Sequence Data</term><term>Molecular Weight</term><term>Nucleocapsid Proteins (analysis)</term><term>Nucleocapsid Proteins (immunology)</term><term>SARS Virus (chemistry)</term><term>Sequence Alignment</term><term>Severe Acute Respiratory Syndrome (diagnosis)</term><term>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization (methods)</term><term>Spike Glycoprotein, Coronavirus</term><term>Viral Envelope Proteins (analysis)</term><term>Viral Envelope Proteins (immunology)</term><term>Viral Proteins (analysis)</term><term>Viral Proteins (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Alignement de séquences</term><term>Animaux</term><term>Données de séquences moléculaires</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires (analyse)</term><term>Glycoprotéines membranaires (immunologie)</term><term>Humains</term><term>Masse moléculaire</term><term>Protéines de l'enveloppe virale (analyse)</term><term>Protéines de l'enveloppe virale (immunologie)</term><term>Protéines nucléocapside (analyse)</term><term>Protéines nucléocapside (immunologie)</term><term>Protéines virales (analyse)</term><term>Protéines virales (immunologie)</term><term>Spectrométrie de masse MALDI ()</term><term>Syndrome respiratoire aigu sévère (diagnostic)</term><term>Séquence d'acides aminés</term><term>Virus du SRAS ()</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Membrane Glycoproteins</term><term>Nucleocapsid Proteins</term><term>Viral Envelope Proteins</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Membrane Glycoproteins</term><term>Nucleocapsid Proteins</term><term>Viral Envelope Proteins</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term><term>Protéines nucléocapside</term><term>Protéines virales</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term><term>Protéines nucléocapside</term><term>Protéines virales</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Humans</term><term>Molecular Sequence Data</term><term>Molecular Weight</term><term>Sequence Alignment</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Alignement de séquences</term><term>Animaux</term><term>Données de séquences moléculaires</term><term>Glycoprotéine de spicule des coronavirus</term><term>Humains</term><term>Masse moléculaire</term><term>Spectrométrie de masse MALDI</term><term>Séquence d'acides aminés</term><term>Virus du SRAS</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A new coronavirus has been implicated as the causative agent of severe acute respiratory syndrome (SARS). We have used convalescent sera from several SARS patients to detect proteins in the culture supernatants from cells exposed to lavage another SARS patient. The most prominent protein in the supernatant was identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) as a approximately 46-kDa species. This was found to be a novel nucleocapsid protein that matched almost exactly one predicted by an open reading frame in the recently published nucleotide sequence of the same virus isolate (>96% coverage). A second viral protein corresponding to the predicted approximately 139-kDa spike glycoprotein has also been examined by MALDI-TOF MS (42% coverage). After peptide N-glycosidase F digestion, 12 glycosylation sites in this protein were confirmed. The sugars attached to four of the sites were also identified. These results suggest that the nucleocapsid protein is a major immunogen that may be useful for early diagnostics, and that the spike glycoprotein may present a particularly attractive target for prophylactic intervention in combating SARS.</div></front></TEI><affiliations><list><country><li>Canada</li></country><region><li>Manitoba</li></region><settlement><li>Winnipeg</li></settlement><orgName><li>Université du Manitoba</li></orgName></list><tree><noCountry><name sortKey="Andonov, Anton" sort="Andonov, Anton" uniqKey="Andonov A" first="Anton" last="Andonov">Anton Andonov</name><name sortKey="Bastien, Nathalie" sort="Bastien, Nathalie" uniqKey="Bastien N" first="Nathalie" last="Bastien">Nathalie Bastien</name><name sortKey="Cheng, Keding" sort="Cheng, Keding" uniqKey="Cheng K" first="Keding" last="Cheng">Keding Cheng</name><name sortKey="Dasuri, Kumar V N" sort="Dasuri, Kumar V N" uniqKey="Dasuri K" first="Kumar V N" last="Dasuri">Kumar V N. Dasuri</name><name sortKey="Ens, Werner" sort="Ens, Werner" uniqKey="Ens W" first="Werner" last="Ens">Werner Ens</name><name sortKey="Feldmann, Heinz" sort="Feldmann, Heinz" uniqKey="Feldmann H" first="Heinz" last="Feldmann">Heinz Feldmann</name><name sortKey="Flick, Ramon" sort="Flick, Ramon" uniqKey="Flick R" first="Ramon" last="Flick">Ramon Flick</name><name sortKey="Jones, Steven" sort="Jones, Steven" uniqKey="Jones S" first="Steven" last="Jones">Steven Jones</name><name sortKey="Li, Yan" sort="Li, Yan" uniqKey="Li Y" first="Yan" last="Li">Yan Li</name><name sortKey="Perreault, Helene" sort="Perreault, Helene" uniqKey="Perreault H" first="Hélène" last="Perreault">Hélène Perreault</name><name sortKey="Plummer, Frank" sort="Plummer, Frank" uniqKey="Plummer F" first="Frank" last="Plummer">Frank Plummer</name><name sortKey="Simonsen, J Neil" sort="Simonsen, J Neil" uniqKey="Simonsen J" first="J Neil" last="Simonsen">J Neil Simonsen</name><name sortKey="Standing, Kenneth G" sort="Standing, Kenneth G" uniqKey="Standing K" first="Kenneth G" last="Standing">Kenneth G. Standing</name><name sortKey="Stroeher, Ute" sort="Stroeher, Ute" uniqKey="Stroeher U" first="Ute" last="Stroeher">Ute Stroeher</name><name sortKey="Wilkins, John" sort="Wilkins, John" uniqKey="Wilkins J" first="John" last="Wilkins">John Wilkins</name></noCountry><country name="Canada"><region name="Manitoba"><name sortKey="Krokhin, Oleg" sort="Krokhin, Oleg" uniqKey="Krokhin O" first="Oleg" last="Krokhin">Oleg Krokhin</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 005F37 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 005F37 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:12775768
|texte= Mass spectrometric characterization of proteins from the SARS virus: a preliminary report.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:12775768" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |